Electrocorticographic and deep intracerebral EEG recording in mice using a telemetry system.
نویسندگان
چکیده
Telemetric EEG recording plays a crucial role in the neurological characterization of various transgenic mouse models giving valuable information about epilepsies and sleep disorders in humans. In the past different experimental approaches have been described using tethered systems and jacket systems containing recorders. A main disadvantage of these is their sometimes unphysiological, restraining character. Telemetric EEG recording overcomes most of these disadvantages and allows precise and highly sensitive measurement under various physiological and pathophysiological conditions and different stages of consciousness, as during seizure activity and different sleep stages. Here we present the first contiguous, detailed description of a successful and quick technique for intraperitoneal implantation or subcutaneous pouch implantation of a radiofrequency transmitter in mice and subsequent lead placement in both epidural and deep intracerebral position. Preoperative preparation of the mice, suitable anesthesia, as well as postoperative treatment including pain management are described in detail to provide optimal postoperative recovery. Finally, we display examples of electrocorticograms and deep intracerebral recordings, present strategies to maximize signal-to-noise ratio, paying special attention to major pitfalls and possible artefacts occurring in telemetric EEG recording in mice.
منابع مشابه
The value of long term EEG monitoring in children: A comparison of ambulatory EEG and video telemetry
PURPOSE Outpatient ambulatory EEG may be followed by inpatient video telemetry EEG when investigating children for possible seizures and for classification of epilepsy. We investigated the value of ambulatory EEG and subsequent video telemetry recording in our centre. METHOD The departmental EEG database was interrogated retrospectively for children undergoing ambulatory recording followed by...
متن کاملLong-term Continuous EEG Monitoring in Small Rodent Models of Human Disease Using the Epoch Wireless Transmitter System
Many progressive neurologic diseases in humans, such as epilepsy, require pre-clinical animal models that slowly develop the disease in order to test interventions at various stages of the disease process. These animal models are particularly difficult to implement in immature rodents, a classic model organism for laboratory study of these disorders. Recording continuous EEG in young animal mod...
متن کاملInnovative Methodology Recording EEG in immature rats with a novel miniature telemetry system
Zayachkivsky A, Lehmkuhle MJ, Fisher JH, Ekstrand JJ, Dudek FE. Recording EEG in immature rats with a novel miniature telemetry system. J Neurophysiol 109: 900–911, 2013. First published October 31, 2012; doi:10.1152/jn.00593.2012.—Serial EEG recordings from immature rat pups are extremely difficult to obtain but important for analyzing animal models of neonatal seizures and other pediatric neu...
متن کاملRecording EEG in immature rats with a novel miniature telemetry system.
Serial EEG recordings from immature rat pups are extremely difficult to obtain but important for analyzing animal models of neonatal seizures and other pediatric neurological conditions as well as normal physiology. In this report, we describe the features and applications of a novel miniature telemetry system designed to record EEG in rat pups as young as postnatal day 6 (P6). First, we have r...
متن کاملUse of Telemetric EEG in Brain Injury
Telemetry technology allows remote measurement and recording of signals such as biopotentials. This technology offers the advantage of long-term EEG recordings without causing unnecessary distress, as happens in EEG systems where implanted leads connect to the recording device through a cable. The EEG recordings can be used to detect changes in the brain activity after a traumatic event. The us...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Brain research. Brain research protocols
دوره 14 3 شماره
صفحات -
تاریخ انتشار 2005